Role of the Zeta Method in Intracytoplasmic Sperm Injection Outcomes in High Sperm DNA Fragmentation in Oligoasthenozoospermic Men
DOI:
https://doi.org/10.31661/gmj.v7i.1107Keywords:
Sperm Injection, Intracytoplasmic, DNA Damage, Male InfertilityAbstract
Background: New methods are needed to optimize intracytoplasmic sperm injection (ICSI) outcomes in oligoasthenozoospermic (OAS) men. We evaluated the level of DNA fragment index (DFI) in OAS men and its impact on ICSI outcomes. In addition, we used the zeta potential method for sperm selection to investigate the efficacy of this technique in improving ICSI outcomes. Materials and Methods: This cross-sectional study was performed on 95 couples. Sperm parameters and sperm DNA fragmentation (SDF) were measured. The couples were divided into the following 3 groups: group I (n=30) where SDF was between 15% and 30%, and routine sperm was selected on the basis of motility and morphology; group II (n=34) where SDF was more than 30%, and the routine sperm selection method was applied on the basis of motility and morphology; and group III (n=31) where SDF was more than 30%, and the sperm selection was performed on the basis of the zeta method. The fertilization rate, embryo development, embryo quality, and implantation rate were evaluated in these 3 groups. Results: The fertilization rate was significantly higher in group I compared with group II (P<0.05). The embryo development rate in group I was significantly higher than that in group II (P<0.001) and group III (P<0.05), and it was significantly lower in group II compared with group III (P<0.05). The embryo quality was higher in group III compared with group II (P<0.01). The implantation rate in group I was significantly higher than that in group II (P<0.05) and group III (P<0.05). Conclusion: The present study indicated that a higher level of SDF has an adverse effect on the ICSI outcome. Furthermore, the zeta potential technique can be a useful method for sperm selection in OAS men. [GMJ.2018;7:e1107]
Â
References
Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: A review of literature. J. Hum. Reproductive Sci. 2015;8(4):191. https://doi.org/10.4103/0974-1208.170370PMid:26752853 PMCid:PMC4691969 Poongothai J, Gopenath T, Manonayaki S. Genetics of human male infertility. Singapore Med J. 2009;50(4):336-47. PMid:19421675 Breznik BP, KovaÄiÄ B, Vlaisavljević V. Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection? Fertil Steril. 2013;99(5):1233-41. https://doi.org/10.1016/j.fertnstert.2012.11.048PMid:23290739 https://doi.org/10.2164/jandrol.110.012005PMid:21088229 Schulte RT, Ohl DA, Sigman M, Smith GD. Sperm DNA damage in male infertility: etiologies, assays, and outcomes. J Assist Reprod Genet. 2010;27(1):3-12. https://doi.org/10.1007/s10815-009-9359-xPMid:20012685 PMCid:PMC2826626 Aydos OS, Yükselten Y, Kaplan F, SunguroÄŸlu A, Aydos K. Analysis of the correlation between sperm DNA integrity and conventional semen parameters in infertile men. Turkish J Urol. 2015;41(4):191. https://doi.org/10.5152/tud.2015.98475PMid:26623148 PMCid:PMC4621147 Simon L, Zini A, Dyachenko A, Ciampi A, Carrell DT. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl. 2017;19(1):80. PMid:27345006 Bonduelle M, Wennerholm U-B, Loft A, Tarlatzis B, Peters C, Henriet S, et al. A multi-centre cohort study of the physical health of 5-year-old children conceived after intracytoplasmic sperm injection, in vitro fertilization and natural conception. Hum Reprod. 2005;20(2):413-9. https://doi.org/10.1093/humrep/deh592PMid:15576393 Dyer S, Chambers G, De Mouzon J, Nygren K, Zegers-Hochschild F, Mansour R, et al. International Committee for Monitoring Assisted Reproductive Technologies world report: assisted reproductive technology 2008, 2009 and 2010. Hum Reprod. 2016;31(7):1588-609. https://doi.org/10.1093/humrep/dew082PMid:27207175 Jakab A, Sakkas D, Delpiano E, Cayli S, Kovanci E, Ward D, et al. Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal aneuploidies. Fertil Steril. 2005;84(6):1665-73. https://doi.org/10.1016/j.fertnstert.2005.05.068PMid:16359962 Cho C-L. Commentary: sperm DNA fragmentation testing in action. Translat Androl Urol. 2017;6(4):S647-S8. https://doi.org/10.21037/tau.2016.11.09PMid:29082193 PMCid:PMC5643604 Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril. 2005;84(4):833-42. https://doi.org/10.1016/j.fertnstert.2004.11.089PMid:16213830 Palermo GD, O'Neill CL, Chow S, Cheung S, Parrella A, Pereira N, et al. Intracytoplasmic sperm injection: state of the art in humans. Reproduction. 2017;154(6):F93-F110. https://doi.org/10.1530/REP-17-0374PMid:29158352 PMCid:PMC5719728 Nasr-Esfahani MH, Razavi S, Vahdati A, Fathi F, Tavalaee M. Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome. J Assist Reprod Genet. 2008;25(5):197-203. https://doi.org/10.1007/s10815-008-9223-4PMid:18483762 PMCid:PMC2582068 Chan PJ, Jacobson JD, Corselli JU, Patton WC. A simple zeta method for sperm selection based on membrane charge. Fertil Steril. 2006;85(2):481-6. https://doi.org/10.1016/j.fertnstert.2005.07.1302PMid:16595231 Zarei-Kheirabadi M, Tavalaee M, Deemeh M, Arabi M, Forouzanfar M, Javadi GR, et al. Evaluation of ubiquitin and annexin V in sperm population selected based on density gradient centrifugation and zeta potential (DGC-Zeta). J Assist Reprod Genet. 2012;29(4):365-71. https://doi.org/10.1007/s10815-011-9689-3PMid:22183502 PMCid:PMC3309980 Esfahani MHN, Deemeh MR, Tavalaee M, Sekhavati MH, Gourabi H. Zeta sperm selection improves pregnancy rate and alters sex ratio in male factor infertility patients: a double-blind, randomized clinical trial. Int J Fertil Steril. 2016;10(2):253. Organisation WH. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction: Cambridge university press; 1999. Jayaraman V, Upadhya D, Narayan PK, Adiga SK. Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage. J Assist Reprod Genet. 2012;29(6):557-63. https://doi.org/10.1007/s10815-012-9742-xPMid:22411295 PMCid:PMC3370043 Parmegiani L, Cognigni GE, Bernardi S, Troilo E, Taraborrelli S, Arnone A, et al. Comparison of two ready-to-use systems designed for sperm–hyaluronic acid binding selection before intracytoplasmic sperm injection: PICSI vs. Sperm Slow: a prospective, randomized trial. Fertil Steril. 2012;98(3):632-7 https://doi.org/10.1016/j.fertnstert.2012.05.043PMid:22749221 https://doi.org/10.1007/BF01133388 Palermo GD, Neri QV, Cozzubbo T, Rosenwaks Z. Perspectives on the assessment of human sperm chromatin integrity. Fertil Steril. 2014;102(6):1508-17. https://doi.org/10.1016/j.fertnstert.2014.10.008PMid:25456796 Chohan KR, Griffin JT, Lafromboise M, Jonge CJ, Carrell DT. Comparison of chromatin assays for DNA fragmentation evaluation in human sperm. J Androl. 2006;27(1):53-9. https://doi.org/10.2164/jandrol.05068PMid:16400078 Yagci A, Murk W, Stronk J, Huszar G. Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange fluorescence study. J Androl. 2010;31(6):566-72. https://doi.org/10.2164/jandrol.109.008912PMid:20133967 https://doi.org/10.1016/j.fertnstert.2008.01.015PMid:18440529 Oehninger S. Clinical management of male infertility in assisted reproduction: ICSI and beyond. Int J Androl. 2011;34(5pt2):e319-29. https://doi.org/10.1111/j.1365-2605.2011.01143.xPMid:21651569 https://doi.org/10.1038/srep39051 Khajavi NA, Razavi S, Mardani M, Tavalaee M, Deemeh MR, Nasr-Esfahani MH. Can Zeta sperm selection method, recover sperm with higher DNA integrity compare to density gradient centrifugation?. Int J Reprod Bio Med 2009;7(2):73-7. Seli E, Gardner DK, Schoolcraft WB, Moffatt O, Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril. 2004;82(2):378-83. https://doi.org/10.1016/j.fertnstert.2003.12.039PMid:15302287 Sakkas D. The use of blastocyst culture to avoid inheritance of an abnormal paternal genome after ICSI. Hum Reprod. 1999;14(1):4-5. https://doi.org/10.1093/humrep/14.1.4PMid:10374083 Duarte C, Nú-ez V, Wong Y, Vivar C, Benites E, Rodriguez U, et al. Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development. JBRA Assist Reprod. 2017;21(4):351. https://doi.org/10.5935/1518-0557.20170055 Zini A, Boman JM, Belzile E, Ciampi A. Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: systematic review and meta-analysis Hum Reprod. Hum Reprod. 2008;23(12):2663-8. https://doi.org/10.1093/humrep/den321PMid:18757447 Wang M, Sun J, Wang L, Gao X, Lu X, Wu Z, et al. Assessment of density gradient centrifugation (DGC) and sperm chromatin dispersion (SCD) measurements in couples with male factor infertility undergoing ICSI. J Assist Reprod Genet. 2014;31(12):1655-63. https://doi.org/10.1007/s10815-014-0339-4PMid:25227693 PMCid:PMC4250460
