Effect of Honey/PVA Hydrogel Loaded by Erythromycin on Full-Thickness Skin Wound Healing in Rats; Stereological Study

Authors

  • Shabnam Movassaghi Department of Anatomical Sciences &Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Zahra Nadia Sharifi Department of Anatomical Sciences &Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Mojtaba Koosha Department of Cellulose and Paper Technology, Faculty of New Technologies Engineering, Shahid Beheshti University, Zirab Campus, Savadkooh, Mazandaran, Iran
  • Mohammad Amin Abdollahifar Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Shahrzad Fathollahipour Department of Chemical and Biomolecular Engineering, University of Akron, Ohio 44325, USA
  • Javad Tavakoli Mechanical Engineering Biomechanics and Implants Research Group, The Medical Device Research Institute (MDRI), School of Computer Science Engineering and Mathematics, Sir Eric Neal Building, Flinders University, Adelaide, Australia
  • Shabnam Abdi Department of Anatomical Sciences &Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

DOI:

https://doi.org/10.31661/gmj.v8i.1362

Keywords:

Erythromycin, Honey, Polyvinyl Alcoho, Wound Healing

Abstract

Background: Skin wounds are a significant public health risk, and treatment of wound remains a challenging clinical problem for medical teams and researchers.Materials and Methods: In the present study, we aimed to investigate the healing effects of honey/polyvinyl alcohol (PVA) hydrogel loaded with erythromycin  as wound dressing on skin wounds in rats, based on histological studies. In this study, 60 male Wistar rats, with a 1.5 ×1.5 cm2 diameter full-thickness wounds on the backs were divided into four groups: honey/PVA with the erythromycin hydrogel group, honey group, PVA group, and the control group, with no treatment. Skin biopsies were prepared at days 4, 7, and 14 for microscopic analyses. The stereological analysis, including the mean area of the wound, length of vessels, numerical density of fibroblast, macrophage, basal cell and volume of the epidermis, dermis, and fibrous tissue were performed. Result: Wounds area in the honey/PVA hydrogel with the erythromycin group were significantly (P<0.05) smaller than in the other group. The numerical density of fibroblast, macrophage, basal cell and volume of the epidermis in the honey/PVA hydrogel with the erythromycin group were significantly higher than other groups.Conclusion: According to our results, honey/PVA hydrogel with erythromycin may promote early wound healing and has a positive influence on fibroblast proliferation and re-epithelialization, and its administration is recommended after further validation of clinical data.

[GMJ. 2019;8:e1362]

References

Martin P. Wound healing--aiming for perfect skin regeneration. Science. 1997;276(5309):75-81.

https://doi.org/10.1126/science.276.5309.75

PMid:9082989

Franz MG, Robson MC, Steed DL, Barbul A, Brem H, Cooper DM, et al. Guidelines to aid healing of acute wounds by decreasing impediments of healing. Wound Repair Regen. 2008;16(6):723-48.

https://doi.org/10.1111/j.1524-475X.2008.00427.x

PMid:19128244

Guo Sa, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-29.

https://doi.org/10.1177/0022034509359125

PMid:20139336 PMCid:PMC2903966

Moulin V. Growth factors in skin wound healing. Eur J Cell Biol. 1995;68(1):1-7.

PMid:8549585

Yang HS, Shin J, Bhang SH, Shin JY, Park J, Im GI, et al. Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma.. Exp Mol Med. 2011:242-.

https://doi.org/10.3858/emm.2011.43.11.070

Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. burns. 2007;33(2):139-48.

https://doi.org/10.1016/j.burns.2006.06.010

PMid:17137719

Miguel SP, Ribeiro MP, Brancal H, Coutinho P, Correia IJ. Thermoresponsive chitosan–agarose hydrogel for skin regeneration. Carbohydr Polym. 2014;111:366-73.

https://doi.org/10.1016/j.carbpol.2014.04.093

PMid:25037363

Morgado PI, Aguiar-Ricardo A, Correia IJ. Asymmetric membranes as ideal wound dressings: An overview on production methods, structure, properties and performance relationship. J Memb Sci. 2015;490:139-51.

https://doi.org/10.1016/j.memsci.2015.04.064

Zhong S, Zhang Y, Lim C. Tissue scaffolds for skin wound healing and dermal reconstruction. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010;2(5):510-25.

https://doi.org/10.1002/wnan.100

PMid:20607703

Valenta C, Auner BG. The use of polymers for dermal and transdermal delivery. Eur J Pharm Biopharm. 2004;58(2):279-89.

https://doi.org/10.1016/j.ejpb.2004.02.017

PMid:15296955

Yeo JH, Lee KG, Kim HC, OH YL, KIM A-J, KIM SY. The Effects of PVA/Chitosan/Fibroin (PCF)-Blended Spongy Sheets on Wound Heailng in Rats. Biol Pharm Bull. 2000;23(10):1220-3.

https://doi.org/10.1248/bpb.23.1220

PMid:11041255

Tamura H, Furuike T, Nair S, Jayakumar R. Biomedical applications of chitin hydrogel membranes and scaffolds. Carbohydr Polym. 2011;84(2):820-4.

https://doi.org/10.1016/j.carbpol.2010.06.001

Holland LC, Norris JM. Medical grade honey in the management of chronic venous leg ulcers. Int J Surg. 2015;20:17-20.

https://doi.org/10.1016/j.ijsu.2015.05.048

PMid:26050953

Cooke J, Dryden M, Patton T, Brennan J, Barrett J. The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide. BMC Res Notes. 2015;8(1):20.

https://doi.org/10.1186/s13104-014-0960-4

PMid:25627827 PMCid:PMC4312449

Burlando B, Cornara L. Honey in dermatology and skin care: a review. J Cosmet Dermatol. 2013;12(4):306-13.

https://doi.org/10.1111/jocd.12058

PMid:24305429

Molan PC. Potential of honey in the treatment of wounds and burns. Am J Clin Dermatol. 2001;2(1):13-9.

https://doi.org/10.2165/00128071-200102010-00003

PMid:11702616

Boateng JS, Matthews KH, Stevens HN, Eccleston GM. Wound healing dressings and drug delivery systems: a review. J J Pharm Sci. 2008;97(8):2892-923.

https://doi.org/10.1002/jps.21210

PMid:17963217

Kar A. Pharmacognosy and pharmacobiotechnology: New Age International; 2003.

Fan Y, Shan-Guang W, Yu-Fang P, Feng-Lan S, Tao L. Preparation and characteristics of erythromycin microspheres for lung targeting. Drug Dev Ind Pharm. 2009;35(6):639-45.

https://doi.org/10.1080/03639040802512243

PMid:19259878

Rafiee S, Nekouyian N, Hosseini S, Sarabandi F, Chavoshi-Nejad M, Mohsenikia M, et al. Effect of Topical Linum usitatissimum on Full Thickness Excisional Skin Wounds. Trauma. Mon. 2017;22(6).

https://doi.org/10.5812/traumamon.39045

Yadollah-Damavandi S, Chavoshi-Nejad M, Jangholi E, Nekouyian N, Hosseini S, Seifaee A , et al.Topical Hypericum perforatumImproves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model. Evid Based Complement Alternat Med. 2015;2015:245328.

https://doi.org/10.1155/2015/245328

PMid:26417372 PMCid:PMC4568368

Gundersen HJG, Jensen EBV, Kieu K, Nielsen J. The efficiency of systematic sampling in stereology—reconsidered. J Microsc. 1999;193(3):199-211.

https://doi.org/10.1046/j.1365-2818.1999.00457.x

PMid:10348656

Pazyar N, Yaghoobi R, Rafiee E, Mehrabian A, Feily A. Skin wound healing and phytomedicine: a review. Skin Pharmacol Physiol. 2014;27(6):303-10.

https://doi.org/10.1159/000357477

PMid:24993834

Abuharfeil N, Al-Oran R, Abo-Shehada M. The effect of bee honey on the proliferative activity of human B-and T-lymphocytes and the activity of phagocytes. Food Agric Immunol. 1999;11(2):169-77.

https://doi.org/10.1080/09540109999843

Majtan J. Honey: an immunomodulator in wound healing. Wound Repair Regen. 2014;22(2):187-92.

https://doi.org/10.1111/wrr.12117

PMid:24612472

Takzaree N, Hassanzadeh G, Rouini MR, Manayi A, Hadjiakhondi A, Zolbin MM. Evaluation of the Effects of Local Application of Thyme Honey in Open Cutaneous Wound Healing. Iran J Public Health. 2017;46(4):545.

PMid:28540272 PMCid:PMC5439045

Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol. 1999;64(3):235-40.

https://doi.org/10.1016/S0378-8741(98)00131-7

Yasasvini S, Anusa R, VedhaHari B, Prabhu P, RamyaDevi D. Topical hydrogel matrix loaded with Simvastatin microparticles for enhanced wound healing activity. Mater Sci Eng C. 2017;72:160-7.

https://doi.org/10.1016/j.msec.2016.11.038

PMid:28024572

Gupta A, Kumar R, Upadhyay N, Surekha P, Roy P. Synthesis, characterization and efficacy of chemically crosslinked PVA hydrogels for dermal wound healing in experimental animals. J Appl Polym Sci. 2009;111(3):1400-8.

https://doi.org/10.1002/app.28990

Published

2019-04-16

Issue

Section

Original Article