Mizaj as an Index in Persian Traditional Medicine Index Could Associate with Sensitivity to the Radiation
DOI:
https://doi.org/10.31661/gmj.v9i.1705Keywords:
Complementary Therapies; Comet Assay; Radiation Tolerance; Traditional; MizajAbstract
Background: The sensitivity to the radiation among human population depends on various parameters. This variation could lead to dissimilar outcome of radiotherapy in similar situations. Mizaj is a well-known term in Persian medicine that present an individualized medicine viewpoint. All of the people will be categorized in cold, moderate, and warm Mizaj. In this study, we aimed to evaluate the possible association between Mizaj and radiosensitivity by comet assay. Materials and Methods: Peripheral blood sample of 30 healthy volunteers (10 cold, 11 moderate and nine warm Mizaj) were taken and divided into two identical parts. The first part was exposed to 4 Gy x-rays, and the second part was regarded as the sham control. Then, DNA damages of samples were evaluated by the neutral comet assay. Results: The results showed that the mean percentage of damaged cells, in all of the irradiated groups including A (warm), B (moderate) and C (cold) was significantly higher than the controls (P<0.001). Moreover, DNA damage rate in the irradiated warm Mizaj group was higher than both cold and moderate irradiated groups, but the difference between moderate and cold irradiated groups was not statistically significant. Conclusion: The results are indicating that warm Mizaj persons could be more radiosensitive than other groups, which their importance in radiotherapy individualization should be evaluated in more extensive studies. [GMJ.2020;9:e1705]
References
Hall EJ, Giaccia AJ. Radiobiology for the Radiologist. Lippincott Williams & Wilkins; 2006. Di Giorgio M, Sardi M, Busto E, Vallerga M, Taja M, Mairal L, editors. Assessment of individual radiosensitivity in human lymphocytes using micronucleus and microgel electrophoresis "Comet" assays. 11th International Congress on the International Radiation Protection Association; 2004. ghasemi S, Shabestani Monfared A, Borzoie sile S, Zabihi E, Amiri M, Abedian S. Predicting Factors of Radiosensitivity in Individual Radiotherapy. J Babol Univ Med Sci. 2015;17(10):67-73. Jongmans W, Hall J. Cellular responses to radiation and risk of breast cancer. Eur J Cancer. 1999;35(4):540-8. https://doi.org/10.1016/S0959-8049(98)00414-6 Chang HW, Kim SY, Yi S-L, Son S-H, Moon SY, Kim JH et al. Expression of Ku80 correlates with sensitivities to radiation in cancer cell lines of the head and neck. Oral Oncol. 2006;42(10):979-86. https://doi.org/10.1016/j.oraloncology.2005.12.016PMid:16472552 Curwen GB, Murphy S, Tawn EJ, Winther JF, Boice JD. A study of DNA damage recognition and repair gene polymorphisms in relation to cancer predisposition and G2 chromosomal radiosensitivity. Environ Mol Mutagen. 2011;52(1):72-6. https://doi.org/10.1002/em.20633PMid:21113933 PMCid:PMC3779426 Pratesi N, Mangoni M, Mancini I, Paiar F, Simi L, Livi L et al. Association between single nucleotide polymorphisms in the XRCC1 and RAD51 genes and clinical radiosensitivity in head and neck cancer. Radiother Oncol. 2011;99(3):356-61. https://doi.org/10.1016/j.radonc.2011.05.062PMid:21704413 Dudley JT, Karczewski KJ. Exploring personal genomics. Oxford University Press; 2013. https://doi.org/10.1093/acprof:oso/9780199644483.001.0001 Mojahedi M, Naseri M, Majdzadeh R, Keshavarz M, Ebadini M, Nazem E et al. Reliability and validity assessment of Mizaj questionnaire: a novel self-report scale in Iranian traditional medicine. Iran Red Crescent Med J. 2014;3 https://doi.org/10.5812/ircmj.15924PMid:24829785 PMCid:PMC4005447 Bhikha R. Tibb and the concept of temperament. J Nat Med. 2005;22:1-4. Ali SM, Alam M. A scientific correlation between blood groups and temperaments in Unani medicine. 2007. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175(1):184-91. https://doi.org/10.1016/0014-4827(88)90265-0 Shahidi M, Mozdarani H, Bryant PE. Radiation sensitivity of leukocytes from healthy individuals and breast cancer patients as measured by the alkaline and neutral comet assay. Cancer Lett. 2007;257(2):263-73. https://doi.org/10.1016/j.canlet.2007.08.002PMid:17881118 Shahidi M, Mozdarani H, Mueller W-U. Radiosensitivity and repair kinetics of gamma-irradiated leukocytes from sporadic prostate cancer patients and healthy individuals assessed by alkaline comet assay. Iran Biomed J. 2010;14(3):67. Köksal G, Dalcà DÖ, Pala FS. Micronuclei in human lymphocytes: the Co-60 gamma-ray dose-response. Mutat Res Genet Toxicol Environ Mutagen. 1996;359(2):151-7. https://doi.org/10.1016/S0165-1161(96)90261-7 Shahabi S, Hassan ZM, Mahdavi M, Dezfouli M, Rahvar MT, Naseri M et al. Hot and Cold natures and some parameters of neuroendocrine and immune systems in traditional Iranian medicine: a preliminary study. J Altern Complement Med. 2008;14(2):147-56. https://doi.org/10.1089/acm.2007.0693PMid:18307392 Abbas AK, Lichtman AH, Pillai S. Basic immunology: functions and disorders of the immune system. Elsevier Health Sciences; 2014. Lu C, Xiao C, Chen G, Jiang M, Zha Q, Yan X et al. Cold and heat pattern of rheumatoid arthritis in traditional Chinese medicine: distinct molecular signatures indentified by microarray expression profiles in CD4-positive T cell. Rheumatol Int. 2012;32(1):61-8. https://doi.org/10.1007/s00296-010-1546-7PMid:20658292 PMCid:PMC3253282
