Dental Pulp Stem Cells Transplantation Improves Passive Avoidance Memory and Neuroinflammation in Trimethyltin-Induced Alzheimer's Disease Rat Model
DOI:
https://doi.org/10.31661/gmj.v10i.2254Keywords:
Stem Cells, Memory, Alzheimer's Disease, Cognitive Dysfunction, TrimethyltinAbstract
Background: According to the increasing incidence of Alzheimer's disease (AD), this study aimed to investigate the effect of dental pulp stem cells (DPSCs) transplantation on passive avoidance memory and neuroinflammation in trimethyltin (TMT)-induced AD rat model. Materials and Methods: In this experimental study, 18 male Wistar rats were randomly divided into three groups: the control rats, TMT+phosphate-buffered saline (PBS) group that received 8 mg/kg TMT plus 0.5 ml PBS, and TMT+DPSCs (TMT+1×106 cells/ml DPSC in 0.5 ml PBS). Then, after one month, a passive avoidance test was performed. Also, nuclear factor kappa-β (NF-Kβ) serum level and the percentage of damaged neurons in the hippocampus were determined. Results: DPSCs transplantation showed significantly increased step-through latency to the dark compartment compared to control and TMT+PBS groups 24 hours aftershock. Also, time spent in the dark compartment of the TMT+DPSCs group significantly decreased compared to control and TMT+PBS groups in 24 and 48 hours after shock (P<0.05). Furthermore, DPSCs transplantation significantly decreased the NF-Kβ serum level and percentage of damaged pyramidal neurons of CA1 compared to the TMT+PBS group (P<0.05). Conclusion: DPSCs transplantation improved memory and learning, regulated NF-Kβ serum level, and decreased damaged neurons of CA1 hippocampus in the TMT-induced AD rat model.
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