Biological Synthesis of Silver Nanoparticles Using Lactobacillus Probiotic Bacterium and Evaluation of Their Cytotoxicity Against Oral Squamous Cell Carcinoma Cell Line: AgNPs Cytotoxicity Against Squamous Cell Carcinoma
AgNPs Cytotoxicity Against Squamous Cell Carcinoma
DOI:
https://doi.org/10.31661/gmj.v12i.2905Keywords:
Biosynthesis, Silver Nanoparticles, Squamous Cell Carcinoma, Cytotoxicity, Anticancer, Lactobacillus AcidophilusAbstract
Background: The applications of nanotechnology have greatly increased in the recent years. Nanotechnology can be used for diagnosis and treatment of many conditions in medicine and dentistry. The aim of this paper is assessment the cytotoxicity of silver nanoparticles (AGNPs) synthesized employing Lactobacillus acidophilus against human oral squamous cell carcinoma (OSCC) cell line. Materials and Methods: In this in vitro, experimental study, AgNPs were biologically synthesized by using L. acidophilus, and characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), ultraviolet-visible (UV-V) spectroscopy and Fourier-transform infrared (FTIR) spectroscopy. The methyl thiazolyl tetrazolium (MTT) test was performed to assess the cytotoxic effects of AgNPs in 3.125, 6.25, 12.5, 25, 50, and 100 μg/mL concentrations within 24 hours. Results: Synthesis of AgNPs was confirmed by visual perception of dark brown color variation (from achromatic) and maximum UV-V absorption at 428 nm. TEM and SEM indicated the spherical form of AgNPs with a median size of 397 nm. FTIR spectroscopy showed the presence of functional groups from the cells involved in the reduction process. The MTT assay indicated that the biosynthesized nanoparticles made a decrease of cell livability in a concentration dependent method. Conclusion: AgNPs produced by Lactobacillus acidophilus have the potential to inhibit OSCC cell line.
References
Edge SB, Compton CC. The American Joint Committee on Cancer. Annals of surgical oncology. 2010;17(6):1471-4.
https://doi.org/10.1245/s10434-010-0985-4
Markopoulos AK. Current aspects on oral squamous cell carcinoma. open dent j. 2012;6:126.
https://doi.org/10.2174/1874210601206010126
Shah JP, Gil Z. Current concepts in management of oral cancer-surgery. Oral oncol. 2009;45(4-5):394-401.
https://doi.org/10.1016/j.oraloncology.2008.05.017
Alsahafi E, Begg K, Amelio I, Raulf N, Lucarelli P, Sauter T, et al. Clinical update on head and neck cancer: molecular biology and ongoing challenges. Cell death dis. 2019;10(8):540.
https://doi.org/10.1038/s41419-019-1769-9
Wu S, Powers S, Zhu W, Hannun YA. Substantial contribution of extrinsic risk factors to cancer development. Nature. 2016;529(7584):43-7.
https://doi.org/10.1038/nature16166
https://doi.org/10.1038/nmat4477
Szymanski MS, Porter RA. Preparation and quality control of silver nanoparticle-antibody conjugate for use in electrochemical immunoassays. J Immunol. 2013;387(1-2):262-9.
https://doi.org/10.1016/j.jim.2012.11.003
Moritz M, Geszke-Moritz M. The newest achievements in synthesis, immobilization and practical applications of antibacterial nanoparticles. Chem Eng J. 2013;228:596-613.
https://doi.org/10.1016/j.cej.2013.05.046
Keshari AK, Srivastava R, Singh P, Yadav VB, Nath G. Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. J Ayurveda Integr Med. 2020;11(1):37-44.
https://doi.org/10.1016/j.jaim.2017.11.003
Rao PV, Nallappan D, Madhavi K, Rahman S, Jun Wei L, Gan SH. Phytochemicals and biogenic metallic nanoparticles as anticancer agents. Oxid Med Cell Longev. 2016;2016:3685671.
https://doi.org/10.1155/2016/3685671
Acharya D, Satapathy S, Somu P, Parida UK, Mishra G. Apoptotic effect and anticancer activity of biosynthesized silver nanoparticles from marine algae Chaetomorpha linum extract against human colon cancer cell HCT-116. Biol Trace Elem Res. 2021;199:1812-22.
https://doi.org/10.1007/s12011-020-02304-7
Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, et al. Green synthesis of silver nanoparticles using Capsicum annuum L. extract Green Chem. 2007;9(8):852-8.
https://doi.org/10.1039/b615357g
Ahmed S, Ahmad M, Swami BL, Ikram S. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res. 2016;7(1):17-28.
https://doi.org/10.1016/j.jare.2015.02.007
Pugazhenthiran N, Anandan S, Kathiravan G, Udaya Prakash NK, Crawford S, Ashokkumar M. Microbial synthesis of silver nanoparticles by Bacillus sp. J Nanopart Res. 2009;11:1811-5.
https://doi.org/10.1007/s11051-009-9621-2
Anjum N, Maqsood S, Masud T, Ahmad A, Sohail A, Momin A. Lactobacillus acidophilus: characterization of the species and application in food production. Crit Rev Food Sci Nutr. 2014;54(9):1241-51.
https://doi.org/10.1080/10408398.2011.621169
Thomas R, Janardhanan A, Varghese RT, Soniya E, Mathew J, Radhakrishnan E. Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp. Braz J Microbiol. 2014;45:1221-7.
https://doi.org/10.1590/S1517-83822014000400012
El-Naggar NE-A, Hussein MH, El-Sawah AA. Bio-fabrication of silver nanoparticles by phycocyanin, characterization, in vitro anticancer activity against breast cancer cell line and in vivo cytotxicity. Sci Rep. 2017;7(1):10844.
https://doi.org/10.1038/s41598-017-11121-3
Baetke SC, Lammers T, Kiessling F. Applications of nanoparticles for diagnosis and therapy of cancer. Br J Radiol. 2015;88(1054):20150207.
https://doi.org/10.1259/bjr.20150207
Hamida RS, Abdelmeguid NE, Ali MA, Bin-Meferij MM, Khalil MI. Synthesis of silver nanoparticles using a novel cyanobacteria Desertifilum sp extract: their antibacterial and cytotoxicity effects. Int J nanomedicine. 2020:49-63.
https://doi.org/10.2147/IJN.S238575
Duong TT, Le TS, Tran TTH, Nguyen TK, Ho CT, Dao TH, et al. Inhibition effect of engineered silver nanoparticles to bloom forming cyanobacteria. Nanoscience and Nanotechnology. 2016;7(3):035018.
https://doi.org/10.1088/2043-6262/7/3/035018
Dos Santos CA, Seckler MM, Ingle AP, Gupta I, Galdiero S, Galdiero M, et al. Silver nanoparticles: therapeutical uses, toxicity, and safety issues. J Pharm Sci. 2014;103(7):1931-44.
https://doi.org/10.1002/jps.24001
Khandel P, Kumar Shahi S, Kanwar L, Kumar Yadaw R, Kumar Soni D. Biochemical profiling of microbes inhibiting Silver nanoparticles using symbiotic organisms. Int j nanodimension. 2018;9(3):273-85.
Inbathamizh L, Ponnu TM, Mary EJ. In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles. J pharm res. 2013;6(1):32-8.
https://doi.org/10.1016/j.jopr.2012.11.010
software informer. spss. united states: software informer; Available from: https://spss.software.informer.com/16.0/2023.
Fulton MD, Najahi-Missaoui W. Liposomes in Cancer Therapy: How Did We Start and Where Are We Now. Int J Mol Sci. 2023;24(7):6615.
https://doi.org/10.3390/ijms24076615
Malik P, Ameta RK, Mukherjee TK. Emerging Drug Delivery Potential of Gold and Silver Nanoparticles to Lung and Breast Cancers In Practical Approach to Mammalian Cell and Organ Culture. Singapore: Springer Nature Singapore; 2023:1109-75.
https://doi.org/10.1007/978-981-19-1731-8_21-2
Missaoui WN, Arnold RD, Cummings BS. Toxicological status of nanoparticles: What we know and what we don't know. Chem Biol Interact. 2018;295:1-12.
https://doi.org/10.1016/j.cbi.2018.07.015
Chang H-I, Yeh M-K. Clinical development of liposome-based drugs: formulation, characterization, and therapeutic efficacy. Int J nanomedicine. 2012:49-60.
https://doi.org/10.2147/IJN.S26766
Anandaradje A, Meyappan V, Kumar I, Sakthivel N. Microbial Synthesis of Silver Nanoparticles and Their Biological Potential In Nanoparticles in Medicine. Singapore Springer Singapore. 2020:99-133.
https://doi.org/10.1007/978-981-13-8954-2_4
Yadav SK. Nanoscale materials in targeted drug delivery. theragnosis and tissue regeneration: Springer; 2016.
https://doi.org/10.1007/978-981-10-0818-4
Raja G, Jang Y-K, Suh J-S, Kim H-S, Ahn SH, Kim T-J. Microcellular Environmental Regulation of Silver Nanoparticles in Cancer Therapy: Critical Review. Cancers. 2020;12(3):664.
https://doi.org/10.3390/cancers12030664
Baran MF, Keskin C, Baran A, Hatipoğlu A, Yildiztekin M, Küçükaydin S, et al. Green Synthesis of Silver Nanoparticles from Allium cepa L Peel Extract, Their Antioxidant, Antipathogenic, and Anticholinesterase Activity. Molecules. 2023;28(5):2310.
https://doi.org/10.3390/molecules28052310
Zhang X-F, Liu Z-G, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci. 2016;17(9):1534.
https://doi.org/10.3390/ijms17091534
Jia Z, Sun H, Gu Q. Preparation of Ag nanoparticles with triethanolamine as reducing agent and their antibacterial property. Colloids and Surfaces A: Physicochem Eng Asp. 2013;419:174-9.
https://doi.org/10.1016/j.colsurfa.2012.12.003
Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B. Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci. 2014;9(6):385.
Mata R, Nakkala JR, Sadras SR. Biogenic silver nanoparticles from Abutilon indicum: Their antioxidant, antibacterial and cytotoxic effects in vitro. Colloids Surf B: Biointerfaces . 2015;128:276-86.
https://doi.org/10.1016/j.colsurfb.2015.01.052
Ovais M, Khalil AT, Ayaz M, Ahmad I, Nethi SK, Mukherjee S. Biosynthesis of metal nanoparticles via microbial enzymes: a mechanistic approach. Int J Mol Sci. 2018;19(12):4100.
https://doi.org/10.3390/ijms19124100
Stone V, Johnston H, Schins RP. Development of in vitro systems for nanotoxicology: methodological considerations. Crit Rev Toxicol. 2009;39(7):613-26.
https://doi.org/10.1080/10408440903120975
Devi J, Bhimba B. Anticancer activity of silver nanoparticles synthesized by the seaweed. Ulva lactuca Invitro. 2012;1:242.
https://doi.org/10.4172/scientificreports.242
Sulaiman GM, Mohammed WH, Marzoog TR, Al-Amiery AAA, Kadhum AAH, Mohamad AB. Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. Asian Pac J Trop Med. 2013;3(1):58-63.
https://doi.org/10.1016/S2221-1691(13)60024-6
Yakop F, Abd Ghafar SA, Yong YK, Saiful Yazan L, Mohamad Hanafiah R, Lim V, et al. Silver nanoparticles Clinacanthus Nutans leaves extract induced apoptosis towards oral squamous cell carcinoma cell lines. Artif Cells Nanomed Biotechnol. 2018;46(sup2):131-9.
https://doi.org/10.1080/21691401.2018.1452750
Rudrappa M, Rudayni HA, Assiri RA, Bepari A, Basavarajappa DS, Nagaraja SK, et al. Plumeria alba-mediated green synthesis of silver nanoparticles exhibits antimicrobial effect and anti-oncogenic activity against glioblastoma U118 MG cancer cell line. Nanomaterials. 2022;12(3):493.
https://doi.org/10.3390/nano12030493
Shah S, Dhawan V, Holm R, Nagarsenker MS, Perrie Y. Liposomes: Advancements and innovation in the manufacturing process. Adv Drug Deliv Rev. 2020;154-155:102-22.
https://doi.org/10.1016/j.addr.2020.07.002
Osouli-Bostanabad K, Puliga S, Serrano DR, Bucchi A, Halbert G, Lalatsa A. Microfluidic Manufacture of Lipid-Based Nanomedicines. Pharmaceutics. 2022;14(9):1940.
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