Cytotoxic and Apoptotic Effects of Vanadyl Sulfate on MCF-7 Breast Cancer Cell Line

Authors

  • Hamid Zaferani Arani Department of Surgery, Shariati Hospital. Tehran University of Medical Sciences, Tehran, Iran
  • Maryam Dehdashti Young Researchers and Elite Club, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Zahra Abbasy Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  • Hesam Adin Atashi Young Researchers and Elite Club, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Seyed Alireza Salimi-Tabatabaee Department of Surgery, KaDepartment of Surgery, Kashan University of Medical Sciences, Kashan, Iranshan University of Medical Sciences, Kashan, Iran
  • Afsaneh Ghasemi Department of Public Health, School of Health, Fasa University of Medical Sciences, Fasa, Iran
  • Zhila Fereidouni Department of Nursing, School of Nursing, Fasa University of Medical Sciences, Fasa, Iran
  • Hadi Zare Marzouni Qaen School of Nursing and Midwifery, Birjand University of Medical Sciences, Birjand, Iran
  • Habib Zakeri Research Center for Neuromodulation and Pain, NAB Pain Clinic, Shiraz University of Medical Sciences, Shiraz, Iran
  • Seyed Abbas Mirmalek Department of Surgery, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

DOI:

https://doi.org/10.31661/gmj.v12i.3050

Keywords:

Vanadyl Sulfate, Breast Cancer, Anti-cancer, MCF-7, Apoptosis, Anti-oxidative

Abstract

Background: Breast cancer (BC) is the major cause of cancer-related death in women. Some studies have indicated the cytotoxic effects of vanadyl oxide sulfate (VOSO4). This study aimed to evaluate the anti-cancer effect of VOSO4 in the treatment of MCF-7 cell lines.
Materials and Methods: The MCF-7 cell line was treated with different concentrations of VOSO4 for 24 and 48 hours. Cell death was measured using the MTT assay. The cell apoptosis rate was measured using Annexin V/Propidium Iodide assay through flow cytometry. Also, the expression levels of p53, P21, Caspase8, superoxide dismutase type 1 (SOD1), Sod2, and Bcl2 mRNAs were assessed, and Western blotting was performed for Sod1 protein.
Results: The results showed that the half-maximal inhibitory concentration (IC50) for VOSO4 was 25 and 20 μg/ml for 24 and 48 hours, respectively. Indeed, VOSO4 has dose-dependent cytotoxic effects on the MCF-7. Also, after exposure to VOSO4 for 24 hours, cell apoptosis reached 52% compared with untreated cells. Moreover, after 24 hours of exposure to VOSO4 with IC50 concentration, the expression of p53, P21, Caspase8, Sod1, and Sod2 mRNAs increased (P<0.05), and the expression of Bcl2 mRNA was decreased (P<0.05). Also, the Western blotting revealed Sod1 protein level markedly increased following exposure to VOSO4 (P<0.05). Conclusion: Our results demonstrated that VOSO4 has an apoptotic and cytotoxic effect on BC cells. Therefore, it could be considered a complementary agent for the medical treatment of patients with BC.

References

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Published

2023-06-21

Issue

Vol. 12 (2023): 2023

Section

Original Article

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https://creativecommons.org/licenses/by/4.0/