Therapeutic Potential of Alpha-pinene in Breast Cancer: Targeting miR-21 and PTEN Gene Expression

Alpha-pinene in Breast Cancer: Targeting miR-21 and PTEN

Authors

  • Mohammad Yazdi 1-Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran/ 2-Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Mahdi Alaee 1-Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran/ 2-Shahid Rajaee Hospital, Qazvin University of Medical Sciences, Qazvin, Iran
  • Shana Ahadi School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Parisa Khanicherag 1-Student Research Committee, Tabriz University of Medical Sciences, Tabriz , Iran/ 2-Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Elham Ghayem Department of Internal Medicine, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Ghasem Farhadi Galil Babadi 1-Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran /2-Department of Clinical Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Fatemeh Ghiasi Instructor of Critical Care Nursing, Department of Anesthesiology, School of Allied Medical Sciences, Ayatollah Taleghani Hospital, Ilam University of Medical Sciences, Ilam, Iran
  • Mojgan Azadpour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Keywords:

Breast Cancer; miR-21; PTEN; Alpha-pinene

Abstract

Background: Alpha-pinene is an organic compound with anticancer properties. This compound has been used as a therapeutic factor in breast cancer (BC). miR-21 causes cancer cell invasion by inhibiting Phosphatase and tensin homolog (PTEN). The present study evaluates the potential effect of Alpha-piene on the expression of PTEN and miR-21 genes in BC cells (MCF-7 cell line). Materials and Methods: In this study, the MCF-7 cell line was used. The cells were treated with Alpha- pinene. The viability of cells with different Alpha-pinene concentrations (i.e., 40, 50, 100, 150, and 200 μM) was evaluated with MTT assay. The expression of PTEN and miR-21 genes was evaluated using RT-qPCR. Results: The survival rate of cells in all concentrations was higher 24 h after treatment compared to 48 h after the treatment (P<0.0001). The expression of miR-21 in cells treated with 100 and 50 μM of Alpha- pinene reduced significantly compared to the control cells(P<0.001). PTEN gene expression was exactly the opposite of miR-21. Therefore, its expression increased significantly in cells treated with 100 μM of Alpha- pinene compared to the control cells (P<0.0001). Conclusion: In general, the use of Alpha- pinene led to decreased and increased expression of miR-21 and PTEN, respectively. These changes lead to the reduction of invasion and proliferation of BC cells. Therefore, the Alpha- pinene combination can be used as a therapeutic strategy to treat patients.

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Published

2024-11-30

How to Cite

Yazdi, M., Alaee , M., Ahadi, S., Khanicherag, P., Ghayem, E., Farhadi Galil Babadi , G., … Azadpour, M. (2024). Therapeutic Potential of Alpha-pinene in Breast Cancer: Targeting miR-21 and PTEN Gene Expression: Alpha-pinene in Breast Cancer: Targeting miR-21 and PTEN . Galen Medical Journal, 13, e3613. Retrieved from https://journals.salviapub.com/index.php/gmj/article/view/3613

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Vol. 13 (2024): 2024: In Progress

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Original Article

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