Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-α5 Gene Expression in Balb/C Mouse Seminiferous Tubules
DOI:
https://doi.org/10.31661/gmj.v8i.1369Keywords:
Collagen Type IV, Laminin, Gene Expression, HyperthyroidismAbstract
Background: Hyperthyroidism is one of the disorders of the thyroid gland, an organ that controls the cellular and molecular behaviors of the seminiferous tubule basement membrane (BM), and ultimately, influences its evolutionary process. We aimed to investigate the effects of hyperthyroidism on immunohistochemical characteristics and gene expression levels of collagen IV and laminin-α5 in seminiferous tubules BM of Balb/C mice. Materials and Methods: Twenty male Balb/C mice were divided into experimental and control groups. The experimental group received 500 mg/l of levothyroxine (L-thyroxine) diluted in drinking water for two months to inducing hyperthyroidism, which was confirmed by radioimmunoassay. At the end of the study, the mice were sacrificed, and their testes were extracted for immunohistochemistry and real-time polymerase chain reaction assays. Results: Although a weak reaction was observed in the experimental specimens, no significant enhancement was noted in color intensity of type IV collagen in the seminiferous tubules BM of the experimental group as compared to the control group (P>0.05). Collagen IV gene expression results in the experimental group were not significantly different from the controls (P>0.05). Thus, there was a significant increase in laminin α5 gene expression compared to the control group (P=0.016). Conclusion: Considering the key role of collagen IV and laminin-α5 in the seminiferous tubule BM in the testes, the results of this study indicated that hyperthyroidism has important effects on both structures and functions of these two components. [GMJ.2019;8:e1369]
References
Tanideh N, Zarifi F, Rafiee S, Khastkhodaei M, Hosseinabadi OK, Tarkesh F, et al. Effect of methanolic extract of corn silk on cisplatin-induced nephrotoxicity in rats. Galen Med J. 2018; 7:1258. Brent GA. Mechanisms of thyroid hormone action. J Clin Investigation. 2012;122(9):3035-43. https://doi.org/10.1172/JCI60047PMid:22945636 PMCid:PMC3433956 Wagner MS, Wajner SM, Maia AL. The role of thyroid hormone in testicular development and function. J Endocrinol. 2008;199(3):351-65. https://doi.org/10.1677/JOE-08-0218PMid:18728126 PMCid:PMC2799043 Singh R, Hamada AJ, Agarwal A. Thyroid hormones in male reproduction and fertility. Open Reprod Sci J. 2011;3:98-104. https://doi.org/10.2174/1874255601103010098 Gheisari F, Pishdad G, Emami M, Behdad K, Karimpour A, Sharifian M. The effect of methimazole on thyroid gland uptake of technetium in hyperthyroid patients. Galen Med. J 2012; 1(1):8-12. Raychoudhury S, Raychoudhury K, Millette C. Biotechnological evaluation of extracellular matrix proteins expressed by cultured testicular cells. J Biotech Res. 2011;3:62-71. Pahang H, Nikravesh MR, Jalali M, Bideskan AE, Zargari P, Nabavi AS. Fibronectin regulation by vitamin C treatment in kidneys of nicotinic mice offspring. Iran Red Crescent Med J. 2014; 16(7):e17056. https://doi.org/10.5812/ircmj.17056PMid:25237577 PMCid:PMC4166096 Amerion M, Tahajjodi S, Hushmand Z, Shahri NM, Nikravesh MR, Jalali M. The effect of maternal thyroid disorders (hypothyroidism and hyperthyroidism) during pregnancy and lactation on skin development in wistar rat newborns. Iran J Basic Med Sci. 2013;16(5):665. Houshang R, Reza NM, Mehdi J. Pattern of laminin expression during kidney morphogenesis in Balb/c mice. Pak J Biol Sci. 2010;13(19):961-5. https://doi.org/10.3923/pjbs.2010.961.965PMid:21313920 Sankiewicz A, Romanowicz L, Laudanski P, Zelazowska-Rutkowska B, Puzan B, Cylwik B, et al. SPR imaging biosensor for determination of laminin-5 as a potential cancer marker in biological material. Analyt Bioanalytical Chem. 2016;408(19):5269-76. https://doi.org/10.1007/s00216-016-9621-xPMid:27209594 PMCid:PMC4925691 Siu MK, Cheng CY. Extracellular matrix and its role in spermatogenesis. Molecular Mechanisms in Spermatogenesis: Springer; 2009. P. 74-91. https://doi.org/10.1007/978-0-387-09597-4_5PMid:19856163 PMCid:PMC4035910 Pokidysheva E, Boudko S, Vranka J, Zientek K, Maddox K, Moser M, et al. Biological role of prolyl 3-hydroxylation in type IV collagen. Proc Natl Acad Sci U S A. 2014;111(1):161-6. https://doi.org/10.1073/pnas.1307597111PMid:24368846 PMCid:PMC3890855 Oefner C, Sharkey A, Gardner L, Critchley H, Oyen M, Moffett A. Collagen type IV at the fetal-maternal interface. Placenta. 2015;36(1):59-68. https://doi.org/10.1016/j.placenta.2014.10.012PMid:25465704 PMCid:PMC4302218 McCall AS, Cummings CF, Bhave G, Vanacore R, Page-McCaw A, Hudson BG. Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture. Cell. 2014;157(6):1380-92. https://doi.org/10.1016/j.cell.2014.05.009PMid:24906154 PMCid:PMC4144415 Tahajjodi SS, Amerion M, Shahri NM, Jalali M, Nikravesh MR. The effect of maternal nicotine on basement membrane collagen IV of brain microvessels changes in neonatal Balb/C mice. Iran J Reprod Med. 2014; 12(4):275. Ero-Tolliver IA, Hudson BG, Bhave G. The ancient immunoglobulin domains of peroxidasin are required to form sulfilimine cross-links in collagen IV. J Biol Chem. 2015;290(35):21741-8. https://doi.org/10.1074/jbc.M115.673996PMid:26178375 PMCid:PMC4571896 Ferreira E, Silva A, Serakides R, Gomes A, Cassali GD. Model of induction of thyroid dysfunctions in adult female mice. Arquivo Bras Med Veterinária Zootecnia. 2007;59(5):1245-9. https://doi.org/10.1590/S0102-09352007000500022 Ulisse S, Rucci N, Piersanti D, Carosa E, Graziano FM, Pavan A, et al. Regulation by thyroid hormone of the expression of basement membrane components in rat prepubertal Sertoli cells. Endocrinology. 1998;139(2):741-7. https://doi.org/10.1210/endo.139.2.5732PMid:9449648 Amini S, Nikravesh MR, Jalali M, Fazel A, Nabavi AS. Effects of oral administration of sodium nitrite on laminin expression in mice testicular interstitium: an immunohistochemical study. Biom Res. 2017;8(20):8671-6. Aldaghi MR, Jalali M, Nikravesh MR, Fazel A, Sankian M. Comparison of insulin and alpha lipoic acid treatment on laminin expression in sciatic nerve of diabetic rats. J Cell Animal Biol. 2013;7(8):102-8. https://doi.org/10.5897/JCAB2013.0372 Aldaghi MR, Jalali M, Nikravesh MR, Fazel A, Sankian M. Effect of α-lipoic acid on expression of collagen IV of the sciatic nerve of diabetic rats. Res Opin Animal Veterinary Sci. 2012;2(12):576-82. Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical analysis of collagen IV and laminin expression in spontaneous melanoma regression in the melanoma-bearing Libechov minipig. Acta histochemica et cytochemica. 2015;48(1):15-26. https://doi.org/10.1267/ahc.14020PMid:25861134 PMCid:PMC4387259 Mohammadi S, Nikravesh MR, Jalali M, Moeen AA, Karimfar MH. Maternal nicotine induces collagen type IV changes in the mice lung parenchyma and its vessels. Tanaffos 2011;10(2):32. Mahdi KS, Mohammad NR, Mehdi J, Alireza F, Mojtaba S, Bideskan AE. Effects of maternal nicotine exposure on expression of laminin alpha 5 in lung tissue of newborn. Pakistan journal of biological sciences: PJBS. 2012 Dec;15(24):1168-75. https://doi.org/10.3923/pjbs.2012.1168.1175PMid:23755407 Jalali M, Nikravesh MR, Dorafshani MM. Immunohistochemical Evaluation of the Effects of Nitrate in Drinking Water on Laminin Alpha-5 Expression in Rat Renal Glomeruli. Iran Red Crescent Med J. 2016;18(12):e42638. https://doi.org/10.5812/ircmj.42638 Shahebrahimi K, Naseri R, Kalantarian TS, Rahimi M, Najafi F, Derakhshandeh K, et al. Effects of levothyroxine treatment on lipid profile in subclinical hypothyroidism: a randomized clinical trial. Galen Med J. 2015;4(2):72-7.
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